Chloride channels in the eel intestine

Chloride channels in the eel intestine

Patches of freshly isolated epithelial cells from eel Anguilla anguilla intestine bathed by the same solution on both sides in the cell-attached configuration had conductances of 57.0+/-1.8 pS (for positive voltages) and 13.3+/-0.7 pS (for negative voltages) (means +/- s.e.m., N=25). Electrical activity was spontaneous in the cell-attached configuration, but was frequently lost after excision. ...

Bicarbonate Transport Systems in the Intestine of the Seawater Eel

Utilizing a pH-stat method, the rates of mucosal and serosal alkalinization were measured separately in the seawater eel intestine. These two rates were dependent on contralateral HCO3" concentration and were inhibited by contralateral application of DIDS, an inhibitor of HCO3~ transport, indicating that the mucosal and serosal alkalinization are due to HCO3~ secretion and absorption, respectiv...

Chloride channels and tight junctions. Focus on "Expression of the chloride channel ClC-2 in the murine small intestine epithelium".

The chloride channel ClC-2 has been implicated in neonatal airway chloride secretion. To assess its role in secretion by the small intestine, we assessed its subcellular expression in ileal segments obtained from mice and studied the chloride transport properties of this tissue. Chloride secretion across the mucosa of murine ileal segments was assessed in Ussing chambers as negative short-circu...

Effects of cortisol on water transport across the eel intestine.

Transepithelial potential difference (PD) and net water flux were estimated simultaneously in the posterior part of the intestine isolated from Japanese eels with special reference to the role of cortisol in the sea-water adaptation of the intestine. When cortisol was previously injected into freshwater eels, the serosa negativity of the PD, water flux and osmotic permeability (Po) increased at...

The reaction of tris-(choline chloride) phosphate with eel cholinesterase.